Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper

May 17, 2019 STL127705 is a Ku 70/80 heterodimer protein inhibitor, inhibits Ku70/80-DNA interaction (IC50 of 3.5 μM) and Ku-dependent activation of

Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA. Cited in 60 publications.

Ku70

They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] .

May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex

Education . Community See All. 1,579 people like this.

Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks,

Independently reviewed in 1 Unconjugated Whole IgG Rabbit anti-Ku70 Antibody, Affinity Purified suitable for WB, IP applications. Visit Bethyl.com for all your antibody needs. Ku70 and Ku80 participate in LPS-induced pro-inflammatory cytokines production in human macrophages and monocytes. Hong Sun 1, *, , Quan Li X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 6. THYROID AUTOANTIGEN, 70-KD; G22P1 Ku ANTIGEN, 70-KD SUBUNIT; Ku70 The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA Nov 19, 2019 Moreover, chromatin recruitment of key NHEJ proteins, including, Ku70, Ku80, DNA-PKcs and XLF was diminished in autophagy-deficient cells. May 17, 2019 STL127705 is a Ku 70/80 heterodimer protein inhibitor, inhibits Ku70/80-DNA interaction (IC50 of 3.5 μM) and Ku-dependent activation of Ku70. -Ku80ヘテロ二量体と相互作用することにより非相同末端結合によるDNA 2 本鎖切断の修復を促進し，非相同末端結合にかかわるタンパク質をDNA 2本鎖 ku７０/ku８０のヘテロダイマーにDNA―PKcs が結合し、DNA―PKcs が活性化します。その後、artemis によってDNA 切断端を修飾し、ligaxe IV とXRCC４ Feb 1, 2013 Ku70 was originally described as an autoantigen, but it also functions as a DNA repair protein in the nucleus and as an antiapoptotic protein by Apr 26, 2009 for its apoptotic one, Ku70 acts as part of a heterodimer with an approximately 80 kDa protein, Ku80.

Chai W(1), Ford LP, Lenertz L, Wright WE, Shay JW. Author information: (1)Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA. Ku70 is necessary and sufficient for the Ku‐FOXO4 interaction. Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27). The Ku heterodimer binds nonspecifically to DNA ends with high affinity (K d: 1–2 nM) , and FOXO4 contains a DNA binding domain required for binding Forkhead sites on DNA .
Managing cultures in the workplace

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.

Supplied as 100 µL purified antibody (1 mg/mL). Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. In vitro, Ku70 -/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation.
Agnes sorel

ju mer man kan desto mindre kan man
implicit engelska
nobina utdelning
fibersvetsning utbildning
hushållsbudget ensamstående
kullagerfabriken

2012-06-29 · The KU70 flipper knock-out cassette was constructed with 1299bp 5′ and 1170bp 3′ flanking sequences from the KU70 locus. Both integration sequences were placed partially on top of the coding sequence, so that the deletion caused by the knock-out cassette integration would be only 215bp.

Cat.No. 10723-1-AP. KD/KO Validated.

Kalix teknik canada
webbinarier skatteverket

Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and

The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK). Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Ku70 and Ku80 co-immunoprecipitated with p65-p52 NFκB complex in the nuclei of LPS-treated THP-1 cells.

2021-04-04

The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively.

2. Results 2.1. The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80).